Seasonal patterns of abundance and population variability were determined for Macrosiphum euphorbiae (Thomas), Myzus persicae (Sulzer), and Aphis nasturtii (Kaltenbach) (Hemiptera: Aphididae) in potato plots from weekly samples for 28 years. All species showed a single annual peak, but arrived and reached peak abundance at different times. Population variability (PV, a proportion between 0 and 1) for the week of peak abundance was close to that of other sample weeks and mean seasonal abundance. Based on mid-season abundance, PV of 0.76 for M. persicae differed significantly from 0.80 for A. nasturtii, as well as from 0.59 for M. euphorbiae. A weekly time scale for abundance, initiated at an early stage of plant growth, produced slightly different estimates of PV early and late in the season than a scale centered on peak abundance for each species. PV at the time of invasion differed from estimates for the rest of the summer. The annual abundance used to estimate PV was best determined in the context of aphid life history. Nevertheless, PV provided a robust and precise metric for comparing population variability among the three species, regardless of their seasonal patterns of abundance.