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Moyet, M., H. Morrill, D. Leal Espinal, E. Bernard, and A. Alyokhin. 2023. Early growth patterns of Bacillus cereus on potato substrate in the presence of low densities of black soldier fly larvae. Microorganisms 11(5): 1284. https://doi.org/10.3390/microorganisms11051284

Insect Husbandry

Bacillus cereus is a common and ubiquitous bacterium that can cause foodborne illnesses in humans and other animals. Common methods of contact between foodborne pathogens and their victims include exposure through contaminated food or food containment products. Using larvae of black soldier flies, Hermetia illucens, for biological conversion of wastes into components of animal feeds …

Moyet, M., H. Morrill, D. Leal Espinal, E. Bernard, and A. Alyokhin. 2023. Early growth patterns of Bacillus cereus on potato substrate in the presence of low densities of black soldier fly larvae. Microorganisms 11(5): 1284. https://doi.org/10.3390/microorganisms11051284 Read More »

Kinney, M., M. Moyet, E. Bernard, and A. Alyokhin. 2022. Suppression of methicillin-resistant Staphylococcus aureus and reduction of other bacteria by black soldier fly larvae reared on potato substrate. Microbiology Spectrum 10(5): e02321-22. https://doi.org/10.1128/spectrum.02321-22

Insect Husbandry /

Larvae of black soldier flies, Hermetia illucens, are increasingly used for biological conversion of animal and plant wastes into ingredients of animal feeds on an industrial scale. The presence of pathogenic microorganisms in harvested larvae may be a serious problem for wide-scale adoption of this technology. Fortunately, black soldier fly larvae may have some antimicrobial …

Kinney, M., M. Moyet, E. Bernard, and A. Alyokhin. 2022. Suppression of methicillin-resistant Staphylococcus aureus and reduction of other bacteria by black soldier fly larvae reared on potato substrate. Microbiology Spectrum 10(5): e02321-22. https://doi.org/10.1128/spectrum.02321-22 Read More »

Yoon, K. A., J. H. Kim, R. Nauen, A. Alyokhin, J. M. Clark, and S. H. Lee. 2022. Characterization of molecular and kinetic properties of two acetylcholinesterases from the Colorado potato beetle, Leptinotarsa decemlineata. Pesticide Biochemistry and Physiology 185: 105137. https://doi.org/10.1016/j.pestbp.2022.105137

Insecticides, Microevolution /

The molecular and biochemical properties of two acetylcholinesterases (LdAChE1 and LdAChE2) from the Colorado potato beetle, Leptinotarsa decemlineata, were investigated in this study. Polyacrylamide gel electrophoresis in conjunction with western blotting with LdAChE1- or LdAChE2-specific antibodies suggested that LdAChE1 exists in a soluble form, whereas LdAChE2 exists in both soluble and amphiphilic forms with a …

Yoon, K. A., J. H. Kim, R. Nauen, A. Alyokhin, J. M. Clark, and S. H. Lee. 2022. Characterization of molecular and kinetic properties of two acetylcholinesterases from the Colorado potato beetle, Leptinotarsa decemlineata. Pesticide Biochemistry and Physiology 185: 105137. https://doi.org/10.1016/j.pestbp.2022.105137 Read More »

Pallis, S., A. Alyokhin, B. Manley, T. B. Rodrigues, A. Buzza, E. Barnes, and K. Narva. 2022. Toxicity of a novel dsRNA-based insecticide to the Colorado potato beetle in laboratory and field trials. Pest Management Science 78: 3836-3848.

Insecticides /

BACKGROUND: The Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae) is one of the most notorious pests of the potato, Solanum tuberosum. Potato beetles are capable of developing resistance to various insecticides in relatively few generations. Novel and effective means of controlling Colorado potato beetle populations are constantly required to protect potato crops and prevent loss …

Pallis, S., A. Alyokhin, B. Manley, T. B. Rodrigues, A. Buzza, E. Barnes, and K. Narva. 2022. Toxicity of a novel dsRNA-based insecticide to the Colorado potato beetle in laboratory and field trials. Pest Management Science 78: 3836-3848. Read More »

Erbland, P., A. Alyokhin, and M. Peterson. 2021. An automated incubator for rearing black soldier fly larvae (Hermetia illucens). Transactions of the American Society of Agricultural and Biological Engineers 64: 1989-1997.

Insect Husbandry /

Biological conversion of agricultural wastes into animal feed ingredients using larvae of black soldier fly (Hermetia illucens) is a promising technology that improves the sustainability of agriculture. We designed and tested a prototype automated incubation system for producing black soldier fly larvae. The system consisted of six 50 L plastic bins enclosed on a ventilated …

Erbland, P., A. Alyokhin, and M. Peterson. 2021. An automated incubator for rearing black soldier fly larvae (Hermetia illucens). Transactions of the American Society of Agricultural and Biological Engineers 64: 1989-1997. Read More »

Rodrigues, T. B., S. K Mishra, K. Sridharan, E. R. Barnes, A. Alyokhin, R. Tuttle, W. Kokulapalan, D. Garby, N. J. Skizim, Y. Tang, B. Manley, L. Aulisa, R. D. Flannagan, C. Cobb, and K. E. Narva. 2021. First sprayable double-stranded RNA-based biopesticide product targets proteasome subunit beta type-5 in Colorado potato beetle (Leptinotarsa decemlineata). Frontiers in Plant Science 12:728652. https://doi.org/10.3389/fpls.2021.728652  

Insecticides /

Colorado potato beetle (CPB, Leptinotarsa decemlineata) is a major pest of potato and other solanaceous vegetables in the Northern Hemisphere. The insect feeds on leaves and can completely defoliate crops. Because of the repeated use of single insecticide classes without rotating active ingredients, many chemicals are no longer effective in controlling CPB. Ledprona is a …

Rodrigues, T. B., S. K Mishra, K. Sridharan, E. R. Barnes, A. Alyokhin, R. Tuttle, W. Kokulapalan, D. Garby, N. J. Skizim, Y. Tang, B. Manley, L. Aulisa, R. D. Flannagan, C. Cobb, and K. E. Narva. 2021. First sprayable double-stranded RNA-based biopesticide product targets proteasome subunit beta type-5 in Colorado potato beetle (Leptinotarsa decemlineata). Frontiers in Plant Science 12:728652. https://doi.org/10.3389/fpls.2021.728652   Read More »

Insinga, J.K., A. Alyokhin, J. Hao, T. Ge, N. F. Marangoni, A. Baron. 2021. Dickeya dianthicola is not vectored by two common insect pests of potato. PhytoFrontiers 1: 160-172. https://doi.org/10.1094/PHYTOFR-12-20-0049-R

Abundance Factors / ,

Dickeya dianthicola (Samson) is an important pathogen causing blackleg disease of potato. Previous work suggested that insects might vector species of Pectobacteriaceae between plants but no conclusive work has confirmed this. Green peach aphids (Myzus persicae Sulzer) and Colorado potato beetles (Leptinotarsa decemlineata Say) are aggressive potato pests and related to known vectors of several …

Insinga, J.K., A. Alyokhin, J. Hao, T. Ge, N. F. Marangoni, A. Baron. 2021. Dickeya dianthicola is not vectored by two common insect pests of potato. PhytoFrontiers 1: 160-172. https://doi.org/10.1094/PHYTOFR-12-20-0049-R Read More »

Liu, Y., T. Moural, B.K.S. Koirala, J. Hernandez, Z. Shen, A. Alyokhin, and F. Zhu. 2021. Structural and functional characterization of one unclassified glutathione s-transferase in xenobiotic adaptation of Leptinotarsa decemlineata. International Journal of Molecular Sciences 22: 11921. https://doi.org/10.3390/ijms222111921

Microevolution /

Arthropod Glutathione S-transferases (GSTs) constitute a large family of multifunctional enzymes that are mainly associated with xenobiotic or stress adaptation. GST-mediated xenobiotic adaptation takes place through direct metabolism or sequestration of xenobiotics, and/or indirectly by providing protection against oxidative stress induced by xenobiotic exposure. To date, the roles of GSTs in xenobiotic adaptation in the …

Liu, Y., T. Moural, B.K.S. Koirala, J. Hernandez, Z. Shen, A. Alyokhin, and F. Zhu. 2021. Structural and functional characterization of one unclassified glutathione s-transferase in xenobiotic adaptation of Leptinotarsa decemlineata. International Journal of Molecular Sciences 22: 11921. https://doi.org/10.3390/ijms222111921 Read More »

Popov, S. Ya., A. V. Alyokhin, and E. D. Kiselyov. 2021. Survival of diapausing females of two-spotted spider mite Tetranychus urticae Koch (Acari: Tetranychidae) submerged in water. International Journal of Acarology 47: 564-567.

Abundance Factors

Two-spotted spider mite, Tetranychus urticae Koch, is a ubiquitous and highly polyphagous plant pest with a worldwide distribution. Diapause is essential for its survival in the areas with unfavorable seasonal conditions. We investigated whether female mites overwintering close to the soil surface may be adapted to seasonal flooding. Among the tested mites diapausing on leaf …

Popov, S. Ya., A. V. Alyokhin, and E. D. Kiselyov. 2021. Survival of diapausing females of two-spotted spider mite Tetranychus urticae Koch (Acari: Tetranychidae) submerged in water. International Journal of Acarology 47: 564-567. Read More »

Mishra, S., J. Dee, W. Moar, J. Dufner-Beattie, J. Baum, N. P. Dias, A. Alyokhin, A. Buzza, S.I. Rondon, M. Clough, and S. Menasha. 2021. Selection for high levels of resistance to double-stranded RNA (dsRNA) in Colorado potato beetle (Leptinotarsa decemlineata Say) using non-transgenic foliar delivery. Scientific Reports 11 (2021): 6523. https://doi.org/10.1038/s41598-021-85876-1

Microevolution /

Insecticidal double-stranded RNAs (dsRNAs) silence expression of vital genes by activating the RNA interference (RNAi) mechanism in insect cells. Despite high commercial interest in insecticidal dsRNA, information on resistance to dsRNA is scarce, particularly for dsRNA products with non-transgenic delivery (ex. foliar/topical application) nearing regulatory review. We report the development of the CEAS 300 population …

Mishra, S., J. Dee, W. Moar, J. Dufner-Beattie, J. Baum, N. P. Dias, A. Alyokhin, A. Buzza, S.I. Rondon, M. Clough, and S. Menasha. 2021. Selection for high levels of resistance to double-stranded RNA (dsRNA) in Colorado potato beetle (Leptinotarsa decemlineata Say) using non-transgenic foliar delivery. Scientific Reports 11 (2021): 6523. https://doi.org/10.1038/s41598-021-85876-1 Read More »