Colorado potato beetle

Yoon, K. A., J. H. Kim, R. Nauen, A. Alyokhin, J. M. Clark, and S. H. Lee. 2022. Characterization of molecular and kinetic properties of two acetylcholinesterases from the Colorado potato beetle, Leptinotarsa decemlineata. Pesticide Biochemistry and Physiology 185: 105137. https://doi.org/10.1016/j.pestbp.2022.105137

Insecticides, Microevolution /

The molecular and biochemical properties of two acetylcholinesterases (LdAChE1 and LdAChE2) from the Colorado potato beetle, Leptinotarsa decemlineata, were investigated in this study. Polyacrylamide gel electrophoresis in conjunction with western blotting with LdAChE1- or LdAChE2-specific antibodies suggested that LdAChE1 exists in a soluble form, whereas LdAChE2 exists in both soluble and amphiphilic forms with a …

Yoon, K. A., J. H. Kim, R. Nauen, A. Alyokhin, J. M. Clark, and S. H. Lee. 2022. Characterization of molecular and kinetic properties of two acetylcholinesterases from the Colorado potato beetle, Leptinotarsa decemlineata. Pesticide Biochemistry and Physiology 185: 105137. https://doi.org/10.1016/j.pestbp.2022.105137 Read More »

Pallis, S., A. Alyokhin, B. Manley, T. B. Rodrigues, A. Buzza, E. Barnes, and K. Narva. 2022. Toxicity of a novel dsRNA-based insecticide to the Colorado potato beetle in laboratory and field trials. Pest Management Science 78: 3836-3848.

Insecticides /

BACKGROUND: The Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae) is one of the most notorious pests of the potato, Solanum tuberosum. Potato beetles are capable of developing resistance to various insecticides in relatively few generations. Novel and effective means of controlling Colorado potato beetle populations are constantly required to protect potato crops and prevent loss …

Pallis, S., A. Alyokhin, B. Manley, T. B. Rodrigues, A. Buzza, E. Barnes, and K. Narva. 2022. Toxicity of a novel dsRNA-based insecticide to the Colorado potato beetle in laboratory and field trials. Pest Management Science 78: 3836-3848. Read More »

Rodrigues, T. B., S. K Mishra, K. Sridharan, E. R. Barnes, A. Alyokhin, R. Tuttle, W. Kokulapalan, D. Garby, N. J. Skizim, Y. Tang, B. Manley, L. Aulisa, R. D. Flannagan, C. Cobb, and K. E. Narva. 2021. First sprayable double-stranded RNA-based biopesticide product targets proteasome subunit beta type-5 in Colorado potato beetle (Leptinotarsa decemlineata). Frontiers in Plant Science 12:728652. https://doi.org/10.3389/fpls.2021.728652  

Insecticides /

Colorado potato beetle (CPB, Leptinotarsa decemlineata) is a major pest of potato and other solanaceous vegetables in the Northern Hemisphere. The insect feeds on leaves and can completely defoliate crops. Because of the repeated use of single insecticide classes without rotating active ingredients, many chemicals are no longer effective in controlling CPB. Ledprona is a …

Rodrigues, T. B., S. K Mishra, K. Sridharan, E. R. Barnes, A. Alyokhin, R. Tuttle, W. Kokulapalan, D. Garby, N. J. Skizim, Y. Tang, B. Manley, L. Aulisa, R. D. Flannagan, C. Cobb, and K. E. Narva. 2021. First sprayable double-stranded RNA-based biopesticide product targets proteasome subunit beta type-5 in Colorado potato beetle (Leptinotarsa decemlineata). Frontiers in Plant Science 12:728652. https://doi.org/10.3389/fpls.2021.728652   Read More »

Insinga, J.K., A. Alyokhin, J. Hao, T. Ge, N. F. Marangoni, A. Baron. 2021. Dickeya dianthicola is not vectored by two common insect pests of potato. PhytoFrontiers 1: 160-172. https://doi.org/10.1094/PHYTOFR-12-20-0049-R

Abundance Factors / ,

Dickeya dianthicola (Samson) is an important pathogen causing blackleg disease of potato. Previous work suggested that insects might vector species of Pectobacteriaceae between plants but no conclusive work has confirmed this. Green peach aphids (Myzus persicae Sulzer) and Colorado potato beetles (Leptinotarsa decemlineata Say) are aggressive potato pests and related to known vectors of several …

Insinga, J.K., A. Alyokhin, J. Hao, T. Ge, N. F. Marangoni, A. Baron. 2021. Dickeya dianthicola is not vectored by two common insect pests of potato. PhytoFrontiers 1: 160-172. https://doi.org/10.1094/PHYTOFR-12-20-0049-R Read More »

Liu, Y., T. Moural, B.K.S. Koirala, J. Hernandez, Z. Shen, A. Alyokhin, and F. Zhu. 2021. Structural and functional characterization of one unclassified glutathione s-transferase in xenobiotic adaptation of Leptinotarsa decemlineata. International Journal of Molecular Sciences 22: 11921. https://doi.org/10.3390/ijms222111921

Microevolution /

Arthropod Glutathione S-transferases (GSTs) constitute a large family of multifunctional enzymes that are mainly associated with xenobiotic or stress adaptation. GST-mediated xenobiotic adaptation takes place through direct metabolism or sequestration of xenobiotics, and/or indirectly by providing protection against oxidative stress induced by xenobiotic exposure. To date, the roles of GSTs in xenobiotic adaptation in the …

Liu, Y., T. Moural, B.K.S. Koirala, J. Hernandez, Z. Shen, A. Alyokhin, and F. Zhu. 2021. Structural and functional characterization of one unclassified glutathione s-transferase in xenobiotic adaptation of Leptinotarsa decemlineata. International Journal of Molecular Sciences 22: 11921. https://doi.org/10.3390/ijms222111921 Read More »

Mishra, S., J. Dee, W. Moar, J. Dufner-Beattie, J. Baum, N. P. Dias, A. Alyokhin, A. Buzza, S.I. Rondon, M. Clough, and S. Menasha. 2021. Selection for high levels of resistance to double-stranded RNA (dsRNA) in Colorado potato beetle (Leptinotarsa decemlineata Say) using non-transgenic foliar delivery. Scientific Reports 11 (2021): 6523. https://doi.org/10.1038/s41598-021-85876-1

Microevolution /

Insecticidal double-stranded RNAs (dsRNAs) silence expression of vital genes by activating the RNA interference (RNAi) mechanism in insect cells. Despite high commercial interest in insecticidal dsRNA, information on resistance to dsRNA is scarce, particularly for dsRNA products with non-transgenic delivery (ex. foliar/topical application) nearing regulatory review. We report the development of the CEAS 300 population …

Mishra, S., J. Dee, W. Moar, J. Dufner-Beattie, J. Baum, N. P. Dias, A. Alyokhin, A. Buzza, S.I. Rondon, M. Clough, and S. Menasha. 2021. Selection for high levels of resistance to double-stranded RNA (dsRNA) in Colorado potato beetle (Leptinotarsa decemlineata Say) using non-transgenic foliar delivery. Scientific Reports 11 (2021): 6523. https://doi.org/10.1038/s41598-021-85876-1 Read More »

Klein, C., M. Baker, A. Alyokhin, and D. Mota-Sanchez. 2021. Geographic variation in dominance of spinosad resistance in Colorado potato beetles (Coleoptera: Chrysomelidae). Journal of Economic Entomology 114: 320-325.

Microevolution /

Eastern New York State is frequently the site of Colorado potato beetle (Leptinotarsa decemlineata Say) populations with the highest observed levels of insecticide resistance to a range of active ingredients. The dominance of a resistant phenotype will affect its rate of increase and the potential for management. On organic farms on Long Island, L. decemlineata …

Klein, C., M. Baker, A. Alyokhin, and D. Mota-Sanchez. 2021. Geographic variation in dominance of spinosad resistance in Colorado potato beetles (Coleoptera: Chrysomelidae). Journal of Economic Entomology 114: 320-325. Read More »

Alyokhin, A. and N. Baron. 2021. Increased cannibalism in Colorado potato beetles feeding on non-preferred host plants in laboratory. Entomologia Experimentalis et Applicata 169: 249-254.

Abundance Factors /

Colorado potato beetle, Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae), is an oligophagous herbivore that feeds on several plant species in the family Solanaceae. Cannibalism is common in this species and accounts for a significant part of natural field mortality.We investigated effects of feeding on potato (Solanum tuberosum L., preferred host), tomato (Solanum lycopersicum L., nonpreferred host), …

Alyokhin, A. and N. Baron. 2021. Increased cannibalism in Colorado potato beetles feeding on non-preferred host plants in laboratory. Entomologia Experimentalis et Applicata 169: 249-254. Read More »

Galimberti, A. and A. Alyokhin. 2018. Lethal and sublethal effects of mineral oil on potato pests. Journal of Economic Entomology 111: 1261-1267.

Insecticides, Viruses / ,

Mineral oil is a product used to reduce Potato Virus Y transmission in potato fields. However, there is little information available about other effects that oil may have on insect pests of potato. To better understand how mineral oil affects potato pests, we performed a series of experiments testing the effects of oil on mortality, …

Galimberti, A. and A. Alyokhin. 2018. Lethal and sublethal effects of mineral oil on potato pests. Journal of Economic Entomology 111: 1261-1267. Read More »

Booth, E., A. Alyokhin, and S. Pinatti. 2017. Adult cannibalism in an oligophagous herbivore, the Colorado potato beetle. Insect Science 24: 295-302.

Abundance Factors /

Cannibalism, or intraspecific predation, can play a major role in changing individual fitness and population processes. In insects, cannibalism frequently occurs across life stages, with cannibals consuming a smaller or more vulnerable stage. Predation of adult insects on one another is considered to be uncommon. We investigated adult cannibalism in the Colorado potato beetle, Leptinotarsa …

Booth, E., A. Alyokhin, and S. Pinatti. 2017. Adult cannibalism in an oligophagous herbivore, the Colorado potato beetle. Insect Science 24: 295-302. Read More »