Microevolution

Yoon, K. A., J. H. Kim, R. Nauen, A. Alyokhin, J. M. Clark, and S. H. Lee. 2022. Characterization of molecular and kinetic properties of two acetylcholinesterases from the Colorado potato beetle, Leptinotarsa decemlineata. Pesticide Biochemistry and Physiology 185: 105137. https://doi.org/10.1016/j.pestbp.2022.105137

Insecticides, Microevolution /

The molecular and biochemical properties of two acetylcholinesterases (LdAChE1 and LdAChE2) from the Colorado potato beetle, Leptinotarsa decemlineata, were investigated in this study. Polyacrylamide gel electrophoresis in conjunction with western blotting with LdAChE1- or LdAChE2-specific antibodies suggested that LdAChE1 exists in a soluble form, whereas LdAChE2 exists in both soluble and amphiphilic forms with a […]

Yoon, K. A., J. H. Kim, R. Nauen, A. Alyokhin, J. M. Clark, and S. H. Lee. 2022. Characterization of molecular and kinetic properties of two acetylcholinesterases from the Colorado potato beetle, Leptinotarsa decemlineata. Pesticide Biochemistry and Physiology 185: 105137. https://doi.org/10.1016/j.pestbp.2022.105137 Read More »

Liu, Y., T. Moural, B.K.S. Koirala, J. Hernandez, Z. Shen, A. Alyokhin, and F. Zhu. 2021. Structural and functional characterization of one unclassified glutathione s-transferase in xenobiotic adaptation of Leptinotarsa decemlineata. International Journal of Molecular Sciences 22: 11921. https://doi.org/10.3390/ijms222111921

Microevolution /

Arthropod Glutathione S-transferases (GSTs) constitute a large family of multifunctional enzymes that are mainly associated with xenobiotic or stress adaptation. GST-mediated xenobiotic adaptation takes place through direct metabolism or sequestration of xenobiotics, and/or indirectly by providing protection against oxidative stress induced by xenobiotic exposure. To date, the roles of GSTs in xenobiotic adaptation in the

Liu, Y., T. Moural, B.K.S. Koirala, J. Hernandez, Z. Shen, A. Alyokhin, and F. Zhu. 2021. Structural and functional characterization of one unclassified glutathione s-transferase in xenobiotic adaptation of Leptinotarsa decemlineata. International Journal of Molecular Sciences 22: 11921. https://doi.org/10.3390/ijms222111921 Read More »

Mishra, S., J. Dee, W. Moar, J. Dufner-Beattie, J. Baum, N. P. Dias, A. Alyokhin, A. Buzza, S.I. Rondon, M. Clough, and S. Menasha. 2021. Selection for high levels of resistance to double-stranded RNA (dsRNA) in Colorado potato beetle (Leptinotarsa decemlineata Say) using non-transgenic foliar delivery. Scientific Reports 11 (2021): 6523. https://doi.org/10.1038/s41598-021-85876-1

Microevolution /

Insecticidal double-stranded RNAs (dsRNAs) silence expression of vital genes by activating the RNA interference (RNAi) mechanism in insect cells. Despite high commercial interest in insecticidal dsRNA, information on resistance to dsRNA is scarce, particularly for dsRNA products with non-transgenic delivery (ex. foliar/topical application) nearing regulatory review. We report the development of the CEAS 300 population

Mishra, S., J. Dee, W. Moar, J. Dufner-Beattie, J. Baum, N. P. Dias, A. Alyokhin, A. Buzza, S.I. Rondon, M. Clough, and S. Menasha. 2021. Selection for high levels of resistance to double-stranded RNA (dsRNA) in Colorado potato beetle (Leptinotarsa decemlineata Say) using non-transgenic foliar delivery. Scientific Reports 11 (2021): 6523. https://doi.org/10.1038/s41598-021-85876-1 Read More »

Klein, C., M. Baker, A. Alyokhin, and D. Mota-Sanchez. 2021. Geographic variation in dominance of spinosad resistance in Colorado potato beetles (Coleoptera: Chrysomelidae). Journal of Economic Entomology 114: 320-325.

Microevolution /

Eastern New York State is frequently the site of Colorado potato beetle (Leptinotarsa decemlineata Say) populations with the highest observed levels of insecticide resistance to a range of active ingredients. The dominance of a resistant phenotype will affect its rate of increase and the potential for management. On organic farms on Long Island, L. decemlineata

Klein, C., M. Baker, A. Alyokhin, and D. Mota-Sanchez. 2021. Geographic variation in dominance of spinosad resistance in Colorado potato beetles (Coleoptera: Chrysomelidae). Journal of Economic Entomology 114: 320-325. Read More »

Alyokhin, A. and Y. H. Chen. 2017. Adaptation to toxic hosts as a factor in the evolution of insecticide resistance. Current Opinion in Insect Science 21: 33-38.

Microevolution, Reviews

Insecticide resistance is a serious economic problem that jeopardizes sustainability of chemical control of herbivorous insects and related arthropods. It can be viewed as a specific case of adaptation to toxic chemicals, which has been driven in large part, but not exclusively, by the necessity for insect pests to tolerate defensive compounds produced by their

Alyokhin, A. and Y. H. Chen. 2017. Adaptation to toxic hosts as a factor in the evolution of insecticide resistance. Current Opinion in Insect Science 21: 33-38. Read More »

Alyokhin, A. and L. Miller. 2015. Propensity for flying and walking by the Colorado potato beetles treated with imidacloprid. American Journal of Potato Research 92: 138-142.

Insecticides, Microevolution /

The Colorado potato beetle, Leptinotarsa decemlineata (Say) is a very serious pest of potatoes which is highly mobile and capable of rapid evolution of resistance to chemical control. Insect movement, resulting in gene flow between resistant and susceptible populations, is considered to be an important factor affecting the development and spread of insecticide resistance. We investigated the

Alyokhin, A. and L. Miller. 2015. Propensity for flying and walking by the Colorado potato beetles treated with imidacloprid. American Journal of Potato Research 92: 138-142. Read More »

Alyokhin, A., D. Mota-Sanchez, M. Baker, W. E. Snyder, S. Menasha, M. Whalon, G. Dively, and W. F. Moarsi. 2015. Red Queen on a potato field: IPM vs. chemical dependency in Colorado potato beetle control. Pest Management Science 71: 343-356.

Insecticides, Microevolution /

Originally designed to reconcile insecticide applications with biological control, the concept of integrated pest management (IPM) developed into the systems-based judicious and coordinated use of multiple control techniques aimed at reducing pest damage to economically tolerable levels. Chemical control, with scheduled treatments, was the starting point for most management systems in the 1950s. Although chemical

Alyokhin, A., D. Mota-Sanchez, M. Baker, W. E. Snyder, S. Menasha, M. Whalon, G. Dively, and W. F. Moarsi. 2015. Red Queen on a potato field: IPM vs. chemical dependency in Colorado potato beetle control. Pest Management Science 71: 343-356. Read More »

Chen, J., A. Alyokhin, D. Mota-Sanchez, M. Baker and M. Whalon. 2014. Variation in fitness among geographically isolated Colorado potato beetle (Coleoptera: Chrysomelidae) populations. Annals of the Entomological Society of America 107: 128-135.

Abundance Factors, Microevolution /

Colorado potato beetle, Leptinotarsa decemlineata (Say) is a major pest of potatoes in North America, Europe, and parts of Asia. It adapts quickly to adverse environments, thereby limiting the longevity of control strategies. Better understanding of variability among geographically isolated populations of this insect might create an opportunity to customize control techniques for local conditions. We investigated

Chen, J., A. Alyokhin, D. Mota-Sanchez, M. Baker and M. Whalon. 2014. Variation in fitness among geographically isolated Colorado potato beetle (Coleoptera: Chrysomelidae) populations. Annals of the Entomological Society of America 107: 128-135. Read More »

Alyokhin, A., M. Baker, D. Mota-Sanchez, G. Dively, and E. Grafius. 2008. Colorado potato beetle resistance to insecticides. American Journal of Potato Research 85: 395-413.

Insecticides, Microevolution, Reviews /

The Colorado potato beetle, Leptinotarsa decemlineata (Say), is widely regarded as the most important insect defoliator of potatoes. Its current range covers about 16 million km2 in North America, Europe, and Asia and continues to expand. This insect has a complicated and diverse life history, which is well-suited to agricultural environments, and makes it a complex and challenging

Alyokhin, A., M. Baker, D. Mota-Sanchez, G. Dively, and E. Grafius. 2008. Colorado potato beetle resistance to insecticides. American Journal of Potato Research 85: 395-413. Read More »