Insecticides

Pallis, S., A. Alyokhin, B. Manley, T. Rodrigues, E. Barnes, and K. Narva. 2023. Baseline susceptibility to a novel dsRNA-based insecticide across US populations of Colorado potato beetle. Agriculture 2023, 13, 2283. https://doi.org/10.3390/agriculture13122283

The Colorado potato beetle, Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae) is an insect defoliator of the potato, Solanum tuberosum L. This species thrives in agricultural environments because of its flexible and complex life history, as well as its ability to evolve insecticide resistance. As a result, it has become a widely distributed agricultural pest. Ledprona (trade …

Pallis, S., A. Alyokhin, B. Manley, T. Rodrigues, E. Barnes, and K. Narva. 2023. Baseline susceptibility to a novel dsRNA-based insecticide across US populations of Colorado potato beetle. Agriculture 2023, 13, 2283. https://doi.org/10.3390/agriculture13122283 Read More »

Pallis, S., A. Alyokhin, B. Manley, T. Rodrigues, E. Barnes, and K. Narva. 2023. Effects of low doses of a novel dsRNA-based biopesticide (Calantha) on the Colorado potato beetle. Journal of Economic Entomology 116: 456-461

The Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae) is a destructive pest of the cultivated potato, Solanum tuberosum. Members of this species are well-suited to agricultural habitats because of a suite of physiological adaptations and their ability to evolve resistance to multiple insecticides. Recently, a novel double-stranded RNA (dsRNA) insecticide (Calantha, active ingredient ledprona) has …

Pallis, S., A. Alyokhin, B. Manley, T. Rodrigues, E. Barnes, and K. Narva. 2023. Effects of low doses of a novel dsRNA-based biopesticide (Calantha) on the Colorado potato beetle. Journal of Economic Entomology 116: 456-461 Read More »

Yoon, K. A., J. H. Kim, R. Nauen, A. Alyokhin, J. M. Clark, and S. H. Lee. 2022. Characterization of molecular and kinetic properties of two acetylcholinesterases from the Colorado potato beetle, Leptinotarsa decemlineata. Pesticide Biochemistry and Physiology 185: 105137. https://doi.org/10.1016/j.pestbp.2022.105137

The molecular and biochemical properties of two acetylcholinesterases (LdAChE1 and LdAChE2) from the Colorado potato beetle, Leptinotarsa decemlineata, were investigated in this study. Polyacrylamide gel electrophoresis in conjunction with western blotting with LdAChE1- or LdAChE2-specific antibodies suggested that LdAChE1 exists in a soluble form, whereas LdAChE2 exists in both soluble and amphiphilic forms with a …

Yoon, K. A., J. H. Kim, R. Nauen, A. Alyokhin, J. M. Clark, and S. H. Lee. 2022. Characterization of molecular and kinetic properties of two acetylcholinesterases from the Colorado potato beetle, Leptinotarsa decemlineata. Pesticide Biochemistry and Physiology 185: 105137. https://doi.org/10.1016/j.pestbp.2022.105137 Read More »

Pallis, S., A. Alyokhin, B. Manley, T. B. Rodrigues, A. Buzza, E. Barnes, and K. Narva. 2022. Toxicity of a novel dsRNA-based insecticide to the Colorado potato beetle in laboratory and field trials. Pest Management Science 78: 3836-3848.

BACKGROUND: The Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae) is one of the most notorious pests of the potato, Solanum tuberosum. Potato beetles are capable of developing resistance to various insecticides in relatively few generations. Novel and effective means of controlling Colorado potato beetle populations are constantly required to protect potato crops and prevent loss …

Pallis, S., A. Alyokhin, B. Manley, T. B. Rodrigues, A. Buzza, E. Barnes, and K. Narva. 2022. Toxicity of a novel dsRNA-based insecticide to the Colorado potato beetle in laboratory and field trials. Pest Management Science 78: 3836-3848. Read More »

Rodrigues, T. B., S. K Mishra, K. Sridharan, E. R. Barnes, A. Alyokhin, R. Tuttle, W. Kokulapalan, D. Garby, N. J. Skizim, Y. Tang, B. Manley, L. Aulisa, R. D. Flannagan, C. Cobb, and K. E. Narva. 2021. First sprayable double-stranded RNA-based biopesticide product targets proteasome subunit beta type-5 in Colorado potato beetle (Leptinotarsa decemlineata). Frontiers in Plant Science 12:728652. https://doi.org/10.3389/fpls.2021.728652  

Colorado potato beetle (CPB, Leptinotarsa decemlineata) is a major pest of potato and other solanaceous vegetables in the Northern Hemisphere. The insect feeds on leaves and can completely defoliate crops. Because of the repeated use of single insecticide classes without rotating active ingredients, many chemicals are no longer effective in controlling CPB. Ledprona is a …

Rodrigues, T. B., S. K Mishra, K. Sridharan, E. R. Barnes, A. Alyokhin, R. Tuttle, W. Kokulapalan, D. Garby, N. J. Skizim, Y. Tang, B. Manley, L. Aulisa, R. D. Flannagan, C. Cobb, and K. E. Narva. 2021. First sprayable double-stranded RNA-based biopesticide product targets proteasome subunit beta type-5 in Colorado potato beetle (Leptinotarsa decemlineata). Frontiers in Plant Science 12:728652. https://doi.org/10.3389/fpls.2021.728652   Read More »

Popov, S. Ya. and A. Alyokhin. 2019. Gender-specific acaricidal properties and sexual transmission of spirotetramat in two-spotted spider mite (Tetranychidae: Acariformes). Journal of Economic Entomology 112: 2186-2192.

Two-spotted spider mite, Tetranychus urticae Koch (Tetranychidae: Acariformes), is one of the most important agricultural pests in the world. Their populations have a tendency of rapidly developing resistance to acaricides, making it necessary to have a variety of active ingredients for sustainable chemical control of this pest. We investigated acaricidal properties of a relatively new insecticide spirotetramat …

Popov, S. Ya. and A. Alyokhin. 2019. Gender-specific acaricidal properties and sexual transmission of spirotetramat in two-spotted spider mite (Tetranychidae: Acariformes). Journal of Economic Entomology 112: 2186-2192. Read More »

Galimberti, A. and A. Alyokhin. 2018. Lethal and sublethal effects of mineral oil on potato pests. Journal of Economic Entomology 111: 1261-1267.

Mineral oil is a product used to reduce Potato Virus Y transmission in potato fields. However, there is little information available about other effects that oil may have on insect pests of potato. To better understand how mineral oil affects potato pests, we performed a series of experiments testing the effects of oil on mortality, …

Galimberti, A. and A. Alyokhin. 2018. Lethal and sublethal effects of mineral oil on potato pests. Journal of Economic Entomology 111: 1261-1267. Read More »

Chen, J., A. Kitazumi, J. Alpuerto, A. Alyokhin, and B. de los Reyes. 2016. Heat-induced mortality and expression of heat shock proteins in Colorado potato beetles treated with imidacloprid. Insect Science 23: 548-554.

The Colorado potato beetle is an important pest of solanaceous plants in the Northern Hemisphere. Better understanding of its physiological responses to temperature stress and their interactions with still-prevalent chemical control has important implications for the management of this insect. We measured mortality and expression of the Hsp70 heat shock proteins in the Colorado potato …

Chen, J., A. Kitazumi, J. Alpuerto, A. Alyokhin, and B. de los Reyes. 2016. Heat-induced mortality and expression of heat shock proteins in Colorado potato beetles treated with imidacloprid. Insect Science 23: 548-554. Read More »

Alyokhin, A. and L. Miller. 2015. Propensity for flying and walking by the Colorado potato beetles treated with imidacloprid. American Journal of Potato Research 92: 138-142.

The Colorado potato beetle, Leptinotarsa decemlineata (Say) is a very serious pest of potatoes which is highly mobile and capable of rapid evolution of resistance to chemical control. Insect movement, resulting in gene flow between resistant and susceptible populations, is considered to be an important factor affecting the development and spread of insecticide resistance. We investigated the …

Alyokhin, A. and L. Miller. 2015. Propensity for flying and walking by the Colorado potato beetles treated with imidacloprid. American Journal of Potato Research 92: 138-142. Read More »

Alyokhin, A., D. Mota-Sanchez, M. Baker, W. E. Snyder, S. Menasha, M. Whalon, G. Dively, and W. F. Moarsi. 2015. Red Queen on a potato field: IPM vs. chemical dependency in Colorado potato beetle control. Pest Management Science 71: 343-356.

Originally designed to reconcile insecticide applications with biological control, the concept of integrated pest management (IPM) developed into the systems-based judicious and coordinated use of multiple control techniques aimed at reducing pest damage to economically tolerable levels. Chemical control, with scheduled treatments, was the starting point for most management systems in the 1950s. Although chemical …

Alyokhin, A., D. Mota-Sanchez, M. Baker, W. E. Snyder, S. Menasha, M. Whalon, G. Dively, and W. F. Moarsi. 2015. Red Queen on a potato field: IPM vs. chemical dependency in Colorado potato beetle control. Pest Management Science 71: 343-356. Read More »