Insecticides

Narva, K., U. Toprak, A. Alyokhin, R. Groves, J.L. Jurat‐Fuentes, W. Moar, R. Nauen, S. Whipple, and G. Head. 2025. Insecticide resistance management scenarios differ for RNA‐based sprays and traits. Insect Molecular Biology 34: 518-526.

Insecticides, Microevolution /

RNA-based bioinsecticides that comprise a dsRNA active ingredient and function by RNA interference (RNAi) are being commercialised as insecticidal traits in transgeniccrops and as sprayable biopesticides. These RNAi insecticidal technologies are valuable alternatives to conventional chemical insecticides due to their efficacy, high degree ofspecificity and favourable human and environmental safety profiles. As with all pesticides, […]

Narva, K., U. Toprak, A. Alyokhin, R. Groves, J.L. Jurat‐Fuentes, W. Moar, R. Nauen, S. Whipple, and G. Head. 2025. Insecticide resistance management scenarios differ for RNA‐based sprays and traits. Insect Molecular Biology 34: 518-526. Read More »

Alyokhin, A.V., B.M. Rosenthal, D.C. Weber, and M.B. Baker. 2025. Towards a unified approach in managing resistance to vaccines, drugs, and pesticides. Biological Reviews 100: 1067-1082.

Insecticides, Microevolution, Reviews

Everywhere, pests and pathogens evolve resistance to our control efforts, impairing human health and welfare. Developing sustainable solutions to this problem requires working with evolved immune and ecological systems, rather than against these evolutionary forces. We advocate a transdisciplinary approach to resistance based on an evolutionary foundation informed by the concepts of integrated pest management

Alyokhin, A.V., B.M. Rosenthal, D.C. Weber, and M.B. Baker. 2025. Towards a unified approach in managing resistance to vaccines, drugs, and pesticides. Biological Reviews 100: 1067-1082. Read More »

Wenninger, E.J., S.P. DeGrey, J. Insinga, E. Knopf, A. Alyokhin, E. R. Barnes, B. Bradford, R. L. Groves, B. Manley, and J. Piaskowski, 2025. Responses of non-target arthropods to the dsRNA bioinsecticide Calantha™ and conventional insecticides targeting Colorado potato beetle, Leptinotarsa decemlineata (Say). American Journal of Potato Research 102: 129-151.

Abundance Factors, Insecticides /

Management of the Colorado potato beetle (Leptinotarsa decemlineata) is reliant on conventional insecticides that can negatively affect non-target arthropods. Calantha™ (active ingredient: ledprona) is a sprayable double-stranded RNA biopesticide specific for L decemlineata proteasome subunit beta 5 gene that triggers the RNA-interference pathway and is designed to have limited non-target effects. To test this hypothesis,

Wenninger, E.J., S.P. DeGrey, J. Insinga, E. Knopf, A. Alyokhin, E. R. Barnes, B. Bradford, R. L. Groves, B. Manley, and J. Piaskowski, 2025. Responses of non-target arthropods to the dsRNA bioinsecticide Calantha™ and conventional insecticides targeting Colorado potato beetle, Leptinotarsa decemlineata (Say). American Journal of Potato Research 102: 129-151. Read More »

Gao, Y., A. Alyokhin, R. Zhang, G. Smagghe, S.R. Palli, J.L. Jurat-Fuentes, and B.E. Tabashnik. 2024. Proactive resistance management for sustaining the efficacy of RNA interference for pest control. Journal of Economic Entomology 117: 1306-1308.

Insecticides, Microevolution /

Biopesticides based on RNA interference (RNAi) took a major step forward with the first registration of a sprayable RNAi product, which targets the world’s most damaging potato pest. Proactive resistance management is needed to delay the evolution of resistance by pests and sustain the efficacy of RNAi biopesticides.

Gao, Y., A. Alyokhin, R. Zhang, G. Smagghe, S.R. Palli, J.L. Jurat-Fuentes, and B.E. Tabashnik. 2024. Proactive resistance management for sustaining the efficacy of RNA interference for pest control. Journal of Economic Entomology 117: 1306-1308. Read More »

Yan, J., R. Nauen, S. Reitz, A. Alyokhin, J. Zhang, D. Mota-Sanchez, Y. Kim, S. R. Palli, S. I. Rondon, B. A. Nault, and J. L. Jurat-Fuentes, M. S. Crossley, W. E. Snyder, A.M.R. Gatehouse, M. P. Zalucki, B. E. Tabashnik, and Y. Gao. 2024. The new kid on the block in insect pest management: sprayable RNAi goes commercial. SCIENCE CHINA Life Sciences 67: 1766-1768.

Insecticides /

No abstract available, please email me for a reprint.

Yan, J., R. Nauen, S. Reitz, A. Alyokhin, J. Zhang, D. Mota-Sanchez, Y. Kim, S. R. Palli, S. I. Rondon, B. A. Nault, and J. L. Jurat-Fuentes, M. S. Crossley, W. E. Snyder, A.M.R. Gatehouse, M. P. Zalucki, B. E. Tabashnik, and Y. Gao. 2024. The new kid on the block in insect pest management: sprayable RNAi goes commercial. SCIENCE CHINA Life Sciences 67: 1766-1768. Read More »

Pallis, S., A. Alyokhin, B. Manley, T. Rodrigues, E. Barnes, and K. Narva. 2023. Baseline susceptibility to a novel dsRNA-based insecticide across US populations of Colorado potato beetle. Agriculture 2023, 13, 2283. https://doi.org/10.3390/agriculture13122283

Insecticides /

The Colorado potato beetle, Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae) is an insect defoliator of the potato, Solanum tuberosum L. This species thrives in agricultural environments because of its flexible and complex life history, as well as its ability to evolve insecticide resistance. As a result, it has become a widely distributed agricultural pest. Ledprona (trade

Pallis, S., A. Alyokhin, B. Manley, T. Rodrigues, E. Barnes, and K. Narva. 2023. Baseline susceptibility to a novel dsRNA-based insecticide across US populations of Colorado potato beetle. Agriculture 2023, 13, 2283. https://doi.org/10.3390/agriculture13122283 Read More »

Pallis, S., A. Alyokhin, B. Manley, T. Rodrigues, E. Barnes, and K. Narva. 2023. Effects of low doses of a novel dsRNA-based biopesticide (Calantha) on the Colorado potato beetle. Journal of Economic Entomology 116: 456-461

Insecticides /

The Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae) is a destructive pest of the cultivated potato, Solanum tuberosum. Members of this species are well-suited to agricultural habitats because of a suite of physiological adaptations and their ability to evolve resistance to multiple insecticides. Recently, a novel double-stranded RNA (dsRNA) insecticide (Calantha, active ingredient ledprona) has

Pallis, S., A. Alyokhin, B. Manley, T. Rodrigues, E. Barnes, and K. Narva. 2023. Effects of low doses of a novel dsRNA-based biopesticide (Calantha) on the Colorado potato beetle. Journal of Economic Entomology 116: 456-461 Read More »

Yoon, K. A., J. H. Kim, R. Nauen, A. Alyokhin, J. M. Clark, and S. H. Lee. 2022. Characterization of molecular and kinetic properties of two acetylcholinesterases from the Colorado potato beetle, Leptinotarsa decemlineata. Pesticide Biochemistry and Physiology 185: 105137. https://doi.org/10.1016/j.pestbp.2022.105137

Insecticides, Microevolution /

The molecular and biochemical properties of two acetylcholinesterases (LdAChE1 and LdAChE2) from the Colorado potato beetle, Leptinotarsa decemlineata, were investigated in this study. Polyacrylamide gel electrophoresis in conjunction with western blotting with LdAChE1- or LdAChE2-specific antibodies suggested that LdAChE1 exists in a soluble form, whereas LdAChE2 exists in both soluble and amphiphilic forms with a

Yoon, K. A., J. H. Kim, R. Nauen, A. Alyokhin, J. M. Clark, and S. H. Lee. 2022. Characterization of molecular and kinetic properties of two acetylcholinesterases from the Colorado potato beetle, Leptinotarsa decemlineata. Pesticide Biochemistry and Physiology 185: 105137. https://doi.org/10.1016/j.pestbp.2022.105137 Read More »

Pallis, S., A. Alyokhin, B. Manley, T. B. Rodrigues, A. Buzza, E. Barnes, and K. Narva. 2022. Toxicity of a novel dsRNA-based insecticide to the Colorado potato beetle in laboratory and field trials. Pest Management Science 78: 3836-3848.

Insecticides /

BACKGROUND: The Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae) is one of the most notorious pests of the potato, Solanum tuberosum. Potato beetles are capable of developing resistance to various insecticides in relatively few generations. Novel and effective means of controlling Colorado potato beetle populations are constantly required to protect potato crops and prevent loss

Pallis, S., A. Alyokhin, B. Manley, T. B. Rodrigues, A. Buzza, E. Barnes, and K. Narva. 2022. Toxicity of a novel dsRNA-based insecticide to the Colorado potato beetle in laboratory and field trials. Pest Management Science 78: 3836-3848. Read More »

Rodrigues, T. B., S. K Mishra, K. Sridharan, E. R. Barnes, A. Alyokhin, R. Tuttle, W. Kokulapalan, D. Garby, N. J. Skizim, Y. Tang, B. Manley, L. Aulisa, R. D. Flannagan, C. Cobb, and K. E. Narva. 2021. First sprayable double-stranded RNA-based biopesticide product targets proteasome subunit beta type-5 in Colorado potato beetle (Leptinotarsa decemlineata). Frontiers in Plant Science 12:728652. https://doi.org/10.3389/fpls.2021.728652  

Insecticides /

Colorado potato beetle (CPB, Leptinotarsa decemlineata) is a major pest of potato and other solanaceous vegetables in the Northern Hemisphere. The insect feeds on leaves and can completely defoliate crops. Because of the repeated use of single insecticide classes without rotating active ingredients, many chemicals are no longer effective in controlling CPB. Ledprona is a

Rodrigues, T. B., S. K Mishra, K. Sridharan, E. R. Barnes, A. Alyokhin, R. Tuttle, W. Kokulapalan, D. Garby, N. J. Skizim, Y. Tang, B. Manley, L. Aulisa, R. D. Flannagan, C. Cobb, and K. E. Narva. 2021. First sprayable double-stranded RNA-based biopesticide product targets proteasome subunit beta type-5 in Colorado potato beetle (Leptinotarsa decemlineata). Frontiers in Plant Science 12:728652. https://doi.org/10.3389/fpls.2021.728652   Read More »