Microevolution

Chen, J., A. Alyokhin, D. Mota-Sanchez, M. Baker and M. Whalon. 2014. Variation in fitness among geographically isolated Colorado potato beetle (Coleoptera: Chrysomelidae) populations. Annals of the Entomological Society of America 107: 128-135.

Colorado potato beetle, Leptinotarsa decemlineata (Say) is a major pest of potatoes in North America, Europe, and parts of Asia. It adapts quickly to adverse environments, thereby limiting the longevity of control strategies. Better understanding of variability among geographically isolated populations of this insect might create an opportunity to customize control techniques for local conditions. We investigated […]

Chen, J., A. Alyokhin, D. Mota-Sanchez, M. Baker and M. Whalon. 2014. Variation in fitness among geographically isolated Colorado potato beetle (Coleoptera: Chrysomelidae) populations. Annals of the Entomological Society of America 107: 128-135. Read More »

Alyokhin, A., M. Baker, D. Mota-Sanchez, G. Dively, and E. Grafius. 2008. Colorado potato beetle resistance to insecticides. American Journal of Potato Research 85: 395-413.

The Colorado potato beetle, Leptinotarsa decemlineata (Say), is widely regarded as the most important insect defoliator of potatoes. Its current range covers about 16 million km2 in North America, Europe, and Asia and continues to expand. This insect has a complicated and diverse life history, which is well-suited to agricultural environments, and makes it a complex and challenging

Alyokhin, A., M. Baker, D. Mota-Sanchez, G. Dively, and E. Grafius. 2008. Colorado potato beetle resistance to insecticides. American Journal of Potato Research 85: 395-413. Read More »

Baker, M. B., A. Alyokhin, A. H. Porter, D. N. Ferro, S. R. Dastur , and N. Galal. 2007. Persistence and inheritance of costs of resistance to imidacloprid in Colorado potato beetle. Journal of Economic Entomology 100: 1871-1879.

Reduced fitness among resistant versus susceptible individuals slows resistance evolution and makes it easier to manage. A loss of resistance costs could indicate novel adaptations or mutations contributing to resistance. We measured costs of resistance to imidacloprid in a Massachusetts resistant population compared with a Massachusetts susceptible population in 1999 in terms of fecundity, hatching

Baker, M. B., A. Alyokhin, A. H. Porter, D. N. Ferro, S. R. Dastur , and N. Galal. 2007. Persistence and inheritance of costs of resistance to imidacloprid in Colorado potato beetle. Journal of Economic Entomology 100: 1871-1879. Read More »

Alyokhin, A., G. Dively, M. Patterson, C. Castaldo, D. Rogers, M. Mahoney, and J. Wollam. 2007. Resistance and cross-resistance to imidacloprid and thiamethoxam in the Colorado potato beetle. Pest Management Science 63: 32-41.

One of the major challenges in managing the Colorado potato beetle, Leptinotarsa decemlineata (Say) is its remarkable ability to develop insecticide resistance to virtually every chemical that has ever been used against it. Resistance is particularly common throughout northeastern U.S. as far north as Maine. The first instances of resistance to imidacloprid have been already reported from

Alyokhin, A., G. Dively, M. Patterson, C. Castaldo, D. Rogers, M. Mahoney, and J. Wollam. 2007. Resistance and cross-resistance to imidacloprid and thiamethoxam in the Colorado potato beetle. Pest Management Science 63: 32-41. Read More »

Alyokhin, A., G. Dively, M. Patterson, D. Rogers, M. Mahoney, and J. Wollam. 2006. Susceptibility of imidacloprid-resistant Colorado potato beetles to non-neonicotinoid insecticides in the laboratory and field trials. American Journal of Potato Research 83: 485-494.

Repeated use of neonicotinoid insecticides has resulted in the first reported cases of Colorado potato beetle (Leptinotarsa decemlineata (Say)) resistance to imidacloprid. In the laboratory we determined susceptibility of the imidacloprid-resistant Colorado potato beetles from a population in Southern Maine to other insecticides currently registered for use on potato. This population was about 30-fold resistant to

Alyokhin, A., G. Dively, M. Patterson, D. Rogers, M. Mahoney, and J. Wollam. 2006. Susceptibility of imidacloprid-resistant Colorado potato beetles to non-neonicotinoid insecticides in the laboratory and field trials. American Journal of Potato Research 83: 485-494. Read More »

Baker, M.B., Alyokhin, A., S. R. Dastur, A. H. Porter, and D. N. Ferro. 2005. Sperm precedence in the overwintered Colorado potato beetles (Coleoptera: Chrysomelidae) and its implications for insecticide resistance management. Annals of the Entomological Society of America 98: 989-995.

Colorado potato beetle, Leptinotarsa decemlineata (Say), is the most important insect defoliator of potatoes and is infamous for its ability to develop insecticide resistance. Sperm precedence is an important consideration in developing resistance management plans. We determined the precedence of sperm from a postdiapause spring mating by using irradiated sterile males. We also investigated whether spring mating

Baker, M.B., Alyokhin, A., S. R. Dastur, A. H. Porter, and D. N. Ferro. 2005. Sperm precedence in the overwintered Colorado potato beetles (Coleoptera: Chrysomelidae) and its implications for insecticide resistance management. Annals of the Entomological Society of America 98: 989-995. Read More »

Alyokhin, A. V., D. N. Ferro, C. W. Hoy, and G. Head. 1999. Laboratory assessment of flight activity displayed by the Colorado potato beetles (Coleoptera: Chrysomelidae) fed on transgenic and Cry3A toxin-treated potato foliage. Journal of Economic Entomology92: 115-120.

Adults of full-sib Colorado potato beetle families were separated into three groups fed on different diets: transgenic potato foliage, potato foliage treated with a foliar formulation of B. thuringiensis endotoxin (12.37 ?g of Cry3A d-endotoxin per 1?l of mixture), and untreated potato foliage. After feeding for 3 hours, all the beetles were placed on a computer-linked flight mill system.

Alyokhin, A. V., D. N. Ferro, C. W. Hoy, and G. Head. 1999. Laboratory assessment of flight activity displayed by the Colorado potato beetles (Coleoptera: Chrysomelidae) fed on transgenic and Cry3A toxin-treated potato foliage. Journal of Economic Entomology92: 115-120. Read More »

Alyokhin, A.V. and D. N. Ferro. 1999. Electrophoretic confirmation of sperm mixing in mated Colorado potato beetles (Coleoptera: Chrysomelidae). Annals of the Entomological Society of America 92: 230-235.

Sperm precedence in the Colorado potato beetle, Leptinotarsa decemlineata (Say), was investigated using phosphoglucomutase (Pgm) allozymes as genetic markers. Females were mated to two males carrying different Pgm alleles, and the proportion of offspring sired by each male was determined by cellulose acetate gel electrophoresis. Our results indicate that Pgm alleles in the Colorado potato beetle are

Alyokhin, A.V. and D. N. Ferro. 1999. Electrophoretic confirmation of sperm mixing in mated Colorado potato beetles (Coleoptera: Chrysomelidae). Annals of the Entomological Society of America 92: 230-235. Read More »